Explanation Of The Results Of The TPHA (Treponemal Palidum Hemagglutination Assay) Examination?

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I want to ask … 4 days ago I did MCU. And the results are non-reactive STS (RPR), TPPA (TPHA) 1:80 (-) What do you mean by this … are my bodies really healthy or have a disease … ?? ?Thank you for your answer.

1 Answer:

Hello Mey Pyeong Hwa,


RPR (rapid plasma reagin) is a non-specific treponema test that detects antibodies (immunoglobulins) for fat material contained in Treponema pallidum cells. However, this examination can also provide positive results in other acute infections and also chronic diseases such as autoimmune diseases. Therefore the reactive RPR results are not necessarily syphilis and must be confirmed by treponemal specific assessment, for example TPHA examination.


TPHA (Treponemal palidum hemagglutination assay) is one treponemal test for the examination of syphilis. TPHA examination detects and assesses antibody titers against Treponema palllidum (the bacteria that causes syphilis). TPHA test can show positive results for life even though syphilis treatment has been successful, so TPHA test cannot prove active infection or syphilis infection that has been treated. TPHA results are documented as negative or positive by including the titer level for example <1/80, 1/80, 1/320, 1/1280. Titers <1/80 (1/2, 1/10, 1/40) are usually interpreted as negative and the subsequent 1/80 titers are interpreted as positive. However, the interpretation for the 1/80 results depends on the laboratory reference as well, whether interpreted as negative or positively weak. Because your interpretation is written negative.


A non-reactive RPR result and a negative TPHA means that you don't have syphilis. If the results of non-reactive RPR and TPHA are positive, it means that you may have been infected with syphilis but are now cured and not in the acute phase of infection. Again the results must be adjusted to the history (presence or absence of syphilis symptoms, history of sexual relations, etc.) and physical examination of the patient. Thus the explanation from me, hopefully useful.

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