The Principles Of The ELFA Method And How It Works?

Illustration of The Principles Of The ELFA Method And How It Works?
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What is the principle of the ELFA method? and how does an HIV test using the ELFA method work?

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Enzyme Linked Fluorescent Assay (ELFA) is a modification of the conventional serologic assay of the Enzym-Linked Immunosorbent Assay (ELISA). The principle of ELFA is the same as ELISA, which is to detect the presence of antibodies using a conjugated enzyme, the peroxidase-antibody conjugate. The tools and reagents it uses are the same as ELISA. The difference between the two lies in the type of substrate used. The substrate used for testing on ELFA is p-hydroxyphenylacetic acid, a fluorescent product that is stable and cannot be affected by light.

HIV-specific antibodies were detectable by ELFA, and the results were confirmed by Western Blot (WB) analysis. The ELFA method with saliva samples gave maximum sensitivity and specificity, for urine samples only had a sensitivity of 95.2% and specificity of 97.4%. This method is more advantageous than ELISA. The ELFA method has a shorter window period, which is 5 days. However, both methods are very accurate and can detect about 95% of infections at 28 days and more than 99.99% of infections at 90 days.


In one study, the ELFA method showed adequate results, this method can reduce the risk of manipulation from the laboratory. The ELFA method has a good level of sensitivity and specificity for diagnosing HIV.

The following articles are related to the ELFA method:


ELFA method for HIV testing


ELFA method for diagnosing HIV


ELFA method

Thus, hopefully this is useful.

dr. Vallensia Nurdiana

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